Brief introduction
This product is used for the extraction, enrichment, and purification steps of genomic nucleic acids in fresh, frozen, or anticoagulant-containing blood samples. The sample is lysed, and nucleic acid is released from the cells under the action of guanidinium salt; the nucleic acid is separated from other impurities in the sample by the specific adsorption of magnetic beads and then rinsed by the washing solution to remove proteins and residual guanidinium salts, etc. Finally, after the elution and magnetic adsorption, we get purified nucleic acid, and the obtained product can be used for PCR amplification, nucleic acid detection and analysis, mutation detection and sequencing, and other experiments.
Advantages
1. For difficult-to-lyse samples (such as whole blood) can also be achieved cleavage combined with one step, without the need to pause in the middle to add other reagents.
2. The extraction process does not need to add proteinase K and can be transported and stored at room temperature.
3.The extracted genomic DNA has good purity, high yield, and complete electrophoretic bands.
4. High extraction efficiency: total rna purification kit the whole extraction process takes about 20 minutes, suitable for 200-400 μL of whole blood sample extraction.
5.Strong applicability can be used with different manufacturers of automated nucleic acid extraction equipment.
Application Fields
1. It is suitable for extracting genomic DNA from fresh, frozen, or anticoagulant-containing blood samples.
2. Suitable for obtaining genomic DNA with high purity from blood samples that are difficult to lyse.